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Étude d'impact sur l'environnement et le milieu social
                                                                                                                                 (Directive : 3214-14-062)
Lithium Guo AO :Projet Moblan Lithium
H357755                                                                                                                                Volume 3 - Annexes

                                                               Relevé des frayères
                                                                          (Hatch, 2019)
                                                    (Document Hatch : H357755-00000-200-066-0011)

                                                                                                                      H357755-00000-123-066-0003, Rév. 0,
                                                                                                                                     Vol. 3 – Annexe XXII
© Hatch 2019 Tous droits réservés, y compris les droits relatifs à l’utilisation de ce document et de son contenu.
Relevé des frayères (Hatch, 2019) - COMEX
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Guo Ao Lithium Ltd.                                                                                                          Project Management Report
Biological Surveys of Moblan Lithium Project                                                          Environment Sustainability and Community Interface
                                                                                                                                          Management
H357755                                                                                                       Aquatic Surveys of Moblan Lithium Project

                                                                   Sommaire exécutif
                                                                    RELEVÉ DES FRAYÈRES

                                                  Rapports d’inventaire biologique Moblan Lithium

                     CONTEXTE

                     Cette étude fait suite au rapport Relevés ichtyologiques (Hatch 2018) dans lequel sont
                     étudiés les habitats et communautés de poissons présents dans les plans d’eau au site du
                     projet minier Moblan Lithium. Pour les détails additionnels concernant le contexte et la
                     description de la zone à l’étude, vous pouvez vous reporter à ce dernier. Les relevés dont il
                     est question dans le présent rapport ont été élaborés pour confirmer et caractériser
                     l'utilisation pour le frai par l'omble de fontaine (Salvelinus fontinalis) de l'habitat potentiel
                     précédemment identifié pour le cours d'eau sans nom # 5. Ces informations contribueront à
                     l’étude d’impact sur l’environnement du projet Moblan Lithium.

                     MÉTHODOLOGIE

                     Tous les relevés ichtyologiques ont été réalisés en partenariat avec EnviroCree Ltd, située
                     dans la municipalité de Mistissini. L'intégration de professionnels de la communauté Crie est
                     un atout étant donné leur connaissance du territoire et de l’utilisation historique des
                     ressources halieutiques dans la région du lac Moblan.

                     Afin de maximiser les chances d'obtenir des résultats, la période post-fraie a été ciblée. Le
                     frai à lieu lorsque la température de l'eau est comprise entre 6 et 11 °C, les femelles pondent
                     de 100 à 5000 œufs dont le diamètre varie entre 3,5 et 5 mm. Pour faciliter le relevé
                     ichtyologique, un permis de gestion de la faune (SEG) a été obtenu du ministère de la Forêt,
                     de la Faune et des Parcs (MFFP) (n ° 2018-10-09-182-10-G-P). Le SEG est disponible à
                     l'annexe A.

                     La sélection des cours d'eau est basée sur les caractérisations effectuées à l'été 2018 lors
                     des inventaires ichtyologiques (Hatch, 2018). Des critères de substrats, de vitesse du
                     courant, de profondeur d'eau, de propreté du substrat et de qualité physico-chimique de l'eau
                     ont été utilisés pour évaluer le potentiel de frai. La présence de l'espèce cible et son stade de
                     maturité ont été pris en compte dans la sélection.

                     RÉSULTATS ET CONCLUSION

                     Les relevés ont confirmé la présence d'œufs d'ombles de fontaine à deux endroits dans le
                     cours d'eau #5, qui était précédemment identifié comme ayant un potentiel élevé. Les
                     caractéristiques physiques et chimiques du cours d'eau, et plus particulièrement les zones de
                     fraie, semblent convenir à l'incubation des œufs et devraient permettre une fraie réussie.

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Guo Ao Lithium Ltd.                                                                                                          Project Management Report
Biological Surveys of Moblan Lithium Project                                                          Environment Sustainability and Community Interface
                                                                                                                                          Management
H357755                                                                                                       Aquatic Surveys of Moblan Lithium Project

                     Les résultats de cet inventaire suggèrent que les cours d’eau qui composent ce système
                     hydrologique sont susceptibles d’offrir un excellent potentiel pour le frai de l'omble de
                     fontaine et de répondre à leurs besoins en termes de croissance, reproduction, alevinage et
                     hivernage. Il est également probable que ces cours d'eau offrent un bon potentiel pour le frai
                     pour d’autres espèces dans les lacs et les étangs de la zone d'étude, ce qui en fait un
                     élément essentiel pour les communautés ichtyologiques. L'omble de fontaine est une espèce
                     commerciale, récréative et de subsistance pour les autochtones partout au Canada, ce qui en
                     fera une espèce clé dans le cadre des discussions avec Pêches et Océans Canada (MPO) et
                     le ministère des Forêts, de la Faune et des Parcs (MFFP).

                     D'après les résultats de cet inventaire, le cours d’eau sans nom # 5 ainsi que toutes les eaux
                     qui composent ce système hydrologique et qui répondent à la définition d'un plan d'eau
                     devraient être considérés comme un habitat du poisson. Toute modification ou destruction de
                     ces habitats nécessitera probablement l'approbation du MPO.

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Guo Ao Lithium Ltd.                                                                                                          Project Management Report
Biological Surveys of Moblan Lithium Project                                                          Environment Sustainability and Community Interface
                                                                                                                                          Management
H357755                                                                                                       Aquatic Surveys of Moblan Lithium Project

                                                                     Table of Contents
1. Introduction ........................................................................................................................................ 1-1

2. Spawning Ichthyological survey methodology .............................................................................. 2-1

       2.1       Selection of water bodies ........................................................................................................... 2-1
       2.2       Habitat characterization ............................................................................................................. 2-2
                 2.2.1 Water quality ................................................................................................................. 2-2
                 2.2.2 Fish egg identification ................................................................................................... 2-2
       2.3       Characteristics of fishing gear ................................................................................................... 2-3
       2.4       Weather Conditions ................................................................................................................... 2-3

3. Survey Results ................................................................................................................................... 3-1
       3.1       Unnamed Stream #5 .................................................................................................................. 3-1

4. Conclusion ......................................................................................................................................... 4-3

5. Bibliography ....................................................................................................................................... 5-1
                                                                               List of Tables
Table 2-1 : Location of the Unnamed watercourse #5 segment ................................................................ 2-2
Table 2-2 : Meteorological observation ...................................................................................................... 2-3
Table 3-1 Physicochemical characteristics of Unnamed Stream #5 ......................................................... 3-2
Table 3-2 Fish habitat characterization of the Unnamed stream #5 ......................................................... 3-2
                                                           List of Figures
Figure 2-1         Kick net ......................................................................................................................... 2-3
Figure B-1         Aerial view of spawning sites of Unnamed stream #5 ..................................................... 2
                                                            List of Photos
Photo 1 Brook trout eggs (Salvelinus fontinalis) ........................................................................................ 3-1
Photo 2 Confirmed spawning site .............................................................................................................. 3-1
Photo 3 Brook trout (Salvelinus fontinalis) egg ......................................................................................... C-1
Photo 4 Measuring a brook trout (Salvelinus fontinalis) egg .................................................................... C-2
Photo 5 Upstream view of Unnamed stream #5 from Unnamed pond #6 ................................................ C-3
Photo 6 Spawning site of Unnamed stream #5......................................................................................... C-4

List of Appendices

Appendix A: Wildlife Management Permit (SEG)

Appendix B: Aerial view of the waterbodies

Appendix C: Photos taken at the site

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Guo Ao Lithium Ltd.                                                                                                          Project Management Report
Biological Surveys of Moblan Lithium Project                                                          Environment Sustainability and Community Interface
                                                                                                                                          Management
H357755                                                                                                       Aquatic Surveys of Moblan Lithium Project

                                                            IMPORTANT NOTICE TO READER

This report was prepared by Hatch Ltd. (“Hatch”) for the exclusive use of Guo Ao Lithium Ltd. (the
“Client”) for the sole purpose of assisting the management of the Client to make decisions with respect to
the Lake Moblan Site (the “Site”), and must not be used for any other purpose, or provided to, relied upon
or used by any other person. Any use of or reliance upon this report by another person is done at their
sole risk and Hatch does not accept any responsibility or liability in connection with that person’s use or
reliance.

This report contains the opinion of Hatch using its professional judgment and reasonable care based
upon observations of the condition of the Site made at the time of preparation of this report, and
information made available to Hatch by the Client or by certain other parties on behalf of the Client (the
“Client or Other Information”).

The use of or reliance upon this report by the Client is subject to the following:

(1) This report is to be read in the context of and subject to the terms of the relevant Purchase Order No.
3464-18-2330 between Hatch and the Client (the “Agreement”), including any methodologies, procedures,
techniques, assumptions and other relevant terms or conditions specified in the Agreement.

(2) This report is meant to be read as a whole, and sections or parts of the report must not be read or
relied upon out of context.

(3) Unless expressly stated otherwise in this report, Hatch has not verified the accuracy, completeness or
validity of the Client or Other Information, makes no representation regarding the accuracy of such
information and does not accept any responsibility or liability in connection with the Client or Other
Information.

(4) The condition, stability and safety of the Structure may change over time (or may have already
changed) due to natural forces or human intervention, and Hatch does not accept any responsibility for
the impact that such changes may have on the accuracy or validity of the opinions, conclusions and
recommendations set out in this report.

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Guo Ao Lithium Ltd.                                                                                                          Project Management Report
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                                                                                                                                          Management
H357755                                                                                                       Aquatic Surveys of Moblan Lithium Project

Working team
Hatch Ltd.

  Name                                                                                        Role
  Management
  Marie-Christine Patoine, ing. M.Sc.A                                                        ESIA Manager
  Elyse Hamel, ing. M.Env.                                                                    Project lead
  Kathleen Vukovics, B.A.                                                                     Environmental surveys lead
  Field surveys
  Jean Novotni, biol.                                                                         Aquatic surveys lead
  Romy Bacon Savard, M.Sc.A.                                                                  Aquatic surveys
  Mapping
  Sladjana Pavlovic, M.Sc.                                                                    GIS

EnviroCree

  Name                                                                                        Role
  Management
  Jim MacLeod                                                                                 Logistical support
  Field surveys
  Jaimee MacLeod (intern)                                                                     Aquatic surveys

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Guo Ao Lithium Ltd.                                                                                                          Project Management Report
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                                                                                                                                          Management
H357755                                                                                                       Aquatic Surveys of Moblan Lithium Project

1.                   Introduction
                     This survey is a follow-up to Hatch’s 2018 Ichthyological survey report that studied existing
                     fish habitats and communities in the water bodies surrounding the Moblan Lithium site. For
                     the background of this study and the description of the area, you can refer to the
                     Ichthyological survey report (Hatch, 2018). The surveys discussed in this report were
                     designed to confirm and characterize spawning use by brook trout (Salvelinus fontinalis) for
                     the previously identified potential habitat within unnamed stream #5.

                     All fishery surveys were carried out in partnership with EnviroCree Ltd. located in the
                     municipality of Mistissinni. The integration of professionals from the Cree community is an
                     asset because of their knowledge of the territory and the historic usage of fisheries resources
                     in the Lac Moblan area.

2.                   Spawning Ichthyological survey methodology
                     To maximize the chances of obtaining results, the post-spawning period was targeted.
                     Spawning occurs when the water temperature is between 6 and 11 °C (Bujold & Vachon,
                     2016). The females will lay from 100 to 5000 eggs ranging between 3.5 and 5 mm in
                     diameter, both number and size of eggs are dependent on the size of the female. Females
                     reach sexual maturity around 3 years of age. Nearing spawning the female digs in the gravel
                     a nest or redd wihtin the sands and gravels where the externally fertilized eggs are deposited.
                     Once fertilized, the eggs are covered by the female and are left unguarded for incubation.
                     The incubation time varies according to the temperature and oxygen concentration of the
                     water. Upon reaching maturity the eggs emerge as fry from the substrate after they have
                     resorbed their yolk sacks (Scott & Crossman, 1974).

                     Water temperatures were reported to below 2°C in similar streams in the region at the end of
                     the October triggering the investigation to occur as eggs would have been expected to be
                     deposited. The use of a spoon shaped, 500 µm mesh kick net, specially designed to extract
                     fish eggs buried in the substrate, was used. The collection consists of gently disturbing the
                     substrate upstream of the net while simultaneously using the flow and net to collect substrate
                     and any deposited eggs in to the net.

                     To facilitate the fisheries investigation, a wildlife management permit (SEG) was obtained
                     from the Ministère de la Forêt, de la Faune et des Parcs (MFFP) (#2018-10-09-182-10-G-P).
                     The SEG is available in Appendix A.

2.1                  Selection of water bodies
                     The selection of the watercourses was based on the characterizations carried out in the
                     summer of 2018 during the ichthyological inventories (Hatch, 2018). A criteria of substrates,

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                     stream gradient, current velocity, water depth, substrate cleanliness and physicochemical
                     water quality were used to assess the spawning potential. In addition, the presence of the
                     target species and its maturity stage were considered in the selection.

                     In additional to Unnamed Stream # 5 meeting physical and chemical requirements the
                     observation of juveniles in the downstream, near unnamed pond #6, indicated successful
                     spawning within the tributary in 2017, further confirming the potential for brook trout spawning
                     in the unnamed stream #5. The location of the selected stream segment is presented in Table
                     2-1.
                                                   Table 2-1 : Location of the Unnamed watercourse #5 segment
                    Waterbody                                            Latitude                  Longitude              UTM (18U)
                                                                                                                    Start
                                                                      Start                  Start                  505242 E
                                                                      50.726242              -74.925734             5619385N
                    Unnamed watercourse #5
                                                                      End                    End                    End
                                                                      50.726807              -74.925351             505269 E
                                                                                                                    5619448 N

2.2                  Habitat characterization
2.2.1                Water quality
                     Surface water quality characteristics were recorded for each spawning site using a YSI Pro
                     Plus multi-parameter probe. The YSI Pro Plus was equipped with temperature (°C),
                     conductivity (µs/cm), dissolved oxygen (mg/L) and pH probes.

2.2.2                Fish egg identification
                     Egg identification was based on diameter, period of the capture and the potential presence of
                     other species. Egg measurements were made using a millimetric ruler.

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2.3                  Characteristics of fishing gear
                     The kick net used had an opening of 30 by 35 cm and a depth of 30 cm with a handle of
                     100 cm. The mesh size of the Nitex® net was 500 µm, See Figure 2-1.

                                                                                     Figure 2-1           Kick net

2.4                  Weather Conditions
                     The survey was post-spawning as previously mentioned when waters were reported to be
Guo Ao Lithium Ltd.                                                                                                          Project Management Report
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3.                   Survey Results
3.1                  Unnamed Stream #5
                     Unnamed stream #5 is characterized as a permanent stream with headwaters at an altitude
                     of 468 meters above sea level (masl) dropping to an elevation of 444 masl. Stream #5
                     originates at the outflow of pond #2 and continues towards and through unnamed pond #6
                     before ultimately discharging into Tortigny Lake. The physicochemical characteristics of the
                     unnamed stream #5 are suitable aquatic life. An aerial view of the water body is presented in
                     Appendix A.

                     In the previous study Brook trout were captured in the watercourse immediately downstream
                     of Pond #2 via minnow trapping and electrofishing (Hatch, 2018). The habitat surrounding the
                     fish capture location is characterized by gravel and sand substrates with clear water with in-
                     stream cover by woody debris. Juvenile brook trout, about 100 individuals, were observed in
                     the downstream section of the stream where it flows into Unnamed pond #6.

                     Visual post spawn surveys identified two potential spawning sites in the downstream section
                     of the unnamed stream #5 adjacent to the unnamed pond #6. These sites were confirmed as
                     spawning sites through egg collections using a kick net.

                             Photo 1 Brook trout eggs (Salvelinus fontinalis)                                      Photo 2 Confirmed spawning site

                     Physicochemical measurements of the water at the two spawning locations, indicated
                     adequate pH and an optimal dissolved oxygen concentration (Morin, 2012). Combining the
                     physical and physicochemical conditions, it is anticipated these sites will be successful
                     incubating the brook trout eggs and complete the spawning cycle. Table 3-1 outlines both the
                     physical and physicochemical characteristics at each confirmed spawning location, while
                     Table 3-2 provides an overview of the entire surveyed section.

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Guo Ao Lithium Ltd.                                                                                                          Project Management Report
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                                               Table 3-1 Physicochemical characteristics of Unnamed Stream #5

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Guo Ao Lithium Ltd.                                                                                                          Project Management Report
Biological Surveys of Moblan Lithium Project                                                          Environment Sustainability and Community Interface
                                                                                                                                          Management
H357755                                                                                                       Aquatic Surveys of Moblan Lithium Project

4.                   Conclusion
                     The surveys confirmed egg deposition of brook trout at two locations within the stream
                     previously identified as having high potential. Both physical and chemical characteristics of
                     the stream and more specifically the spawning areas appear suitable for egg incubation and
                     is expected to result in a successful spawn.

                     The results of this survey suggest that all the waterways that make up this hydrological
                     system are likely to offer excellent potential for brook trout spawning and to support their
                     needs in terms of growth, reproduction, rearing and wintering. It also likely these streams
                     provide spawning opportunities for populations within the lakes and ponds of the study area
                     making them a vital component to the overall fish community. Brook trout are an important
                     commercial, recreational and aboriginal (CRA) species throughout Canada as such will be a
                     key species for discussion with Fisheries and Oceans Canada (DFO) and Ministère des
                     Forêts, de la Faune et des Parcs (MFFP). DFO defines fish habitat as “spawning grounds
                     and any other areas, including nursery, rearing, food supply and migration areas, on which
                     fish depend directly or indirectly in order to carry out their life processes;” Based on the
                     results of this survey Unnamed Stream #5 as well any connecting waters meeting the
                     definition of a waterbody, should be considered fish habitat. Moving forward any alteration or
                     destruction of these habitats will likely require DFO approval.

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5.                   Bibliography
Bujold, J.-N., & Vachon, M. (2016). Guide d’identification de frayères à omble de fontaine dans les cours
         d’eau. Ministère des Forêts, de la Faune et des Parcs.
Hatch. (2018). Report - Ichthyological surveys - H357755-00000-200-066-0001, Rev. A. Montréal: Hatch.
Morin, R. (2012). « Qualité de l’eau requise pour l’élevage des salmonidés ». Document d’information
         DADD-14. 25 p. Ministère de l’Agriculture, des Pêcheries et de l’Alimentation.
         http://www.mapaq.gouv.qc.ca/Fr/Peche.
Scott, W., & Crossman, E. (1974). Poisson d'eau douce du Canada, Bulletin 184. Ottawa: Office des
         recherches sur les pêcheries du Canada.

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                                                    Appendix A
                                         Wildlife Management Permit (SEG)

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Guo Ao Lithium Ltd.                                                                                                          Project Management Report
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                                                   Appendix B
                                         Aerial view of the waterbodies

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                                                    Aerialto view
                                                             the use of
                                                                     ofthis document or its
                                                                         spawning           contents.
                                                                                          sites   of    Unnamed stream #5
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                                                               Appendix C
                                                    Photos taken at the site

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                                                               Photo 3 Brook trout (Salvelinus fontinalis) egg

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                                                     Photo 4 Measuring a brook trout (Salvelinus fontinalis) egg

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                                                                                                                                          Management
H357755                                                                                                       Aquatic Surveys of Moblan Lithium Project

                                           Photo 5 Upstream view of Unnamed stream #5 from Unnamed pond #6

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© Hatch 2019 All rights reserved, including all rights relating to the use of this document or its contents.
Guo Ao Lithium Ltd.                                                                                                          Project Management Report
Biological Surveys of Moblan Lithium Project                                                          Environment Sustainability and Community Interface
                                                                                                                                          Management
H357755                                                                                                       Aquatic Surveys of Moblan Lithium Project

                                                                Photo 6 Spawning site of Unnamed stream #5

                                                                                                                      H357755-00000-200-066-0011, Rev. 0,
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                                                                                                                                                  Ver: 04.03
© Hatch 2019 All rights reserved, including all rights relating to the use of this document or its contents.
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